LiliChro's CPC (Centrifugal Partition Chromatography) Instruments
Technology
CPC is a commonly used chromatography technique in laboratory scale. Biphasic system development is quick and easy. Scale-up is well determined and can be done much faster than in case of conventional prepLC. Cost-effectiveness, industrialization, automation, and solvent recycling have been the key issues of the industrial solution of CPC.
CPC as liquid-liquid chromatography technique and HPLC (high-performance liquid chromatography) are similar in several respects. They share the same fundamental mechanism (separation of solutes), the same goal (separation, purification), and the same ancillary techniques (pumps, injectors, detectors).​
​Process
The simple separation funnel is the perfect analogy for liquid-liquid chromatography methods. The basic rule is that compounds can be separated by their different partition coefficients in a biphasic mixture.
If the compounds look very alike, they are similar, let’s say isomers, epimers, or other – than this separation funnel is not suitable. It will give low separation or low yield. Repeating the extraction can increase the separation, and therefore, yield; however after 3-4 mixing it becomes a boring and complicated process. That’s why liquid-liquid chromatography is invented.
Scalable Instruments for all your needs!
LiliChro has 4 instruments, from mg scale to ton scale to meet all of your needs.
The LLC, in a unique way in chromatography, is very easy to scale up (unlike prepLC). On several occasions we faced problems where we had very little amount of material available. With the smallest device available until now (100ml cell volume), 1-2g of sample is needed for a feasibility study.
The Lilichro, and the world’s smallest instrument, works with a minilili 20ml cell volume and a flow rate of 1-2 ml/min, and is therefore often comparable to a conventional analytical HPLC. This is a big step forward for LLC.
Our prepLLC device, Lilichro prep, on the other hand, has a cell volume of 100 litres – five times larger than the largest unit ever created. The completely redesigned cell system has also greatly increased efficiency, raising the monthly purifiable volume to 0.5-2T.
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Instruments
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MiniLili (mg scale)
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MidiLili (g scale)
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MaxiLili (kg scale)
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PrepLili (ton scale)
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